Overview

Protein Chemistry Core Laboratory Director

Information

Areas of Research Interest

Research Interests

My laboratory is interested in the structure-function relations of the gonadotropins. These are members of the glycoprotein hormone family, which consists of the three gonadotropins, luteinizing hormone (LH), follicle-stimulating hormone (FSH), and chorionic gonadotropin (CG). The fourth member of this protein family is thyroid stimulating hormone (TSH). Together, the glycoprotein hormones form a unique subset of the cystine knot growth factor superfamily. While most other superfamily members are located at the C-terminal end of a larger precursor from which they are cleaved, and the N-terminal portion then acts as a regulatory factor, the glycoprotein subunits consist of only the cystine knot domains. The subunits are dissimilar proteins, a common alpha subunit that is differentially glycosylated in each hormone, as well as a hormone-specific beta subunit. Another unusual facet of glycoprotein hormone structure-function is these hormones are decorated with N-linked and sometimes O-linked glycans, which are necessary for function. The glycans can determine metabolic clearance rates of the hormones and at least some of them are necessary for biological activity, although the mechanism is unknown. Glycosylation varies in two ways: microheterogeneity and macroheterogeneity. The former results from a population of 2-105 glycans found at each N- or O-glycosylation site, while the latter results from partial glycosylation of the hormone-specific beta subunit. In humans an interesting pattern of glycosylation is found in FSH. The alpha subunit is always N-glycosylated, while the FSHΒ subunit appears to be glycosylated in an all-or-none manner. This produces a FSH glycoform that possesses four N-glycans that we call tetra-glycosylated hFSH and another form that possesses only the alpha subunit N-glycans that we call di-glycosylated hFSH. In young women, di-glycosylated hFSH is the more abundant glycoform present in the pituitary gland, however, there appears to be a progressive loss of this glycoform with increasing age. We are interested in determining if this can be used as a marker for reproductive aging.

 

Areas of Teaching Interest

Courses Taught

 

Number Course Name

BIOL 210

General Biology I

BIOL 310

Human Reproduction: Issues and Perspectives

BIOL 626

Reproductive Biology

BIOL 710

Glycobiology

BIOL 767

Mechanisms of Hormone Action

 

Publications

AnchorPublications

  1. Teng, C.T., Teng, C-S., Bousfield, G.R., Liu, W-K., & Ward, D.N. 1982. Differential Response  of Growing and Regressing Chicken Ovaries to Gonadotropic Hormones.  Gen. Comp. Endocrinol., 48, 325-332.
  2. Jirgensens, B., Bousfield, G.R., & Moore, W.T., Jr. 1983. Circular Dichroism and Conformational Properties of Modecin, A Toxic Protein.  J. Protein Chem., 2, 105-112.
  3. Bousfield, G.R., & Ward, D.N. 1984. Purification of Lutropin and Follitropin in High Yield from Horse Pituitary Glands.  J. Biol. Chem.,  259, 1911-1921.
  4. Bousfield, G.R., Liu, W-K., & Ward, D.N. 1985. Hybrids from equine LH: alpha enhances, beta diminishes activity.  Molec. Cell. Endocrinol.,  40, 60-77.
  5. Bousfield, G.R., Sugino, H., & Ward, D.N. 1985. Demonstration of a COOH-terminal Extension on Equine Lutropin by Means of a Common Acid-labile Bond in Equine Lutropin and Chorionic Gonadotropin.  J. Biol. Chem., 260, 9531-9533.
  6. Liu, W-K., Bousfield, G.R., Moore, W.T., Jr., & Ward, D.N. 1985. Priming Procedure and  Hormone Preparations Influence Rat Granulosa Cell Response.  Endocrinology, 116, 1454-1459.
  7. Bousfield, G.R. & Ward, D.N. 1986. Direct Demonstration of Intrinsic Follicle-Stimulating Hormone Receptor-Binding Activity in Acid-treated Equine Luteinizing Hormone.  Biochim. Biophys. Acta., 885, 327-334.
  8. Liehr, J.G., Stancel, G.M., Chorich, L.P., Bousfield, G.R., & Ulubelen, A.A. 1986. Hormonal Carcinognesis:  Separation of Estrogenicity from Carcinogenicity.  Chem.-Biol. Interactions, 59, 173-184.
  9. Sugino, H., Bousfield, G.R., Moore, W.T., Jr., & Ward, D.N. 1987. Structural Studies on Equine Glycoprotein Hormones:  Amino acid Sequence of Equine Chorionic Gonadotropin Beta-Subunit.  J. Biol. Chem.,  262, 8603-8609.
  10. Bousfield, G.R., Liu, W-K., Sugino, H., & Ward, D.N. 1987. Structural Studies on Equine Glycoprotein Hormones:  Amino acid Sequence of Equine Lutropin Beta Subunit.  J. Biol. Chem.,  262, 8610-8620.
  11. Bidart, J-M., Troalen, F., Salesse, Bousfield, G.R., Bohouon, C.J., & Bellet, D.H. 1987. Topographic Antigenic Determinants Recognized by Monoclonal Antibodies on Human Choriogonadotropin Beta-Subunit.  J. Biol. Chem., 262, 8551-8556.
  12. Ward, D.N., Wen, T., & Bousfield, G.R. 1987. Sulfate and Phosphate Analysis in Glycoproteins and Other Biologic Compounds Using Ion Chromatography:  Application to Glycoprotein Hormones and Sugar Esters.  J. Chromatog.,  398,  255-264.
  13. Bidart, J-M., Troalen, F., Bousfield, G.R., Birken, S., & Bellet, D. 1988. Antigenic Determinants on Human Choriogonadotropin Beta-Subunit.  I.  Characterization of Topographic Sites Recognized by Monoclonal Antibodies.  J. Biol. Chem.,  263, 10364-10369.
  14. Bousfield, G.R. & Ward, D.N. 1988. Selective Proteolysis of Ovine Lutropin or its Beta Subunit by Endoproteinase Arg-C:  Properties of the Arg (Β-43) cleaved Hormone.  J. Biol. Chem.,  263, 12602-12607.
  15. Ward, D.N., Bousfield, G.R., Gordon, W.L., & Sugino, H. 1988. Chemistry of the Peptide Components of Glycoprotein Hormones.  In: Keel, B.A. and Grotjan, H.E., Jr., (eds).  Microheterogeneity of Glycoprotein Hormones,  Boca Raton: CRC Press, 1-21. 
  16. Ward, D.N., Hines, K.K., Gordon, W.L. & Bousfield, G.R. 1988. The Purification of Native Inhibin and Chemical Characterization.  In: Hodgen, G.D., Rosenwaks, Z., and  Spieler, J.M., (eds.) Nonsteroidal Gonadal Factors: Physiological Roles and Possibilities in Contraceptive Development, Norfolk: The Jones Institute Press, 1-16.
  17. Bousfield, G. R., Liu, W-K., & Ward, D. N. 1989. Effects of Removal of Carboxyterminal Extension From Equine LH Beta-Subunit.  Endocrinology, 124, 379-387.
  18. Bidart, J., Troalen, F., Bousfield, G. R., Bohuon, C., & Bellet, D. 1989. Monoclonal Antibodies Directed to Human and Equine Chorionic Gonadotropins as Probes for the Topographic Analysis of Epitopes on the Human Beta-Subunit.  Endocrinology, 124, 923-929.
  19. Gordon, W.L., Bousfield, G.R. & Ward, D.N. 1989. Comparative binding of FSH to chicken and rat testis.  J. Endocrinol. Invest., 12, 383-392.
  20. Ward, D.N., Bousfield, G.R., & Mar, A.O. 1989. Chemical Reduction-Reoxidation of the Glycoprotein Hormone Disulfide Bonds.  In: Bellet, D. and  Bidart, J.-M., (eds.) Structure-Function Relationship of Gonadotropins, New York: Raven Press, 1-19.
  21. Moore, K.H., Dunbar, B.S., Bousfield, G.R., & Ward, D.N. 1990. The Heterogeneity of  Porcine 32,000 Mr Inhibin alpha-subunit:  A Gel Electrophoresis and Immunoblot Study.  Endocrinology, 127, 1477-1486.
  22. Ward, D.N., Bousfield, G.R., & Moore, K.H. 1990. Gonadotropins.  In: Cupps, P.T., (ed.) Reproduction in Domestic Animals, 4th ed., Orlando, FL: Academic  Press, 25-80.
  23. Matsui, T., Sugino, H., Miura, M., Bousfield, G.R., Ward, D.N., Titani, K., & Mizouchi, T. 1991. Beta-subunits of equine chorionic gonadotropin and luteinizing hormone with an identical amino acid sequence have different asparagine-linked oligosaccharide chains.  Biochem. Biophys. Res. Commun.,  174, 940-945.
  24. Mizuno M., Farach–Carson M.C., Pinero G.J., Fujisawa R., Brunn J.C., Seyer J.M., Bousfield G.R., Mark M.P., & Butler W.T. 1992. Identification of the rat bone 60K acidic glycoprotein as alpha2HS–glycoprotein. Bone Miner, 13, 1-21.
  25. Bousfield, G.R. & Ward, D.N. 1992. Reduction and reoxidation of equine gonadotropin alpha–subunits. Endocrinology, 131, 2986-2998.
  26. Smith P.L., Bousfield G.R., Kumar S., Fiete D., & Baenziger J.U.  1993. Equine lutropin and chorionic gonadotropin bear oligosaccharides terminating with SO4–4–GalNAc and siaα2,3Gal respectively. J. Biol. Chem., 268, 795-802.
  27. Couture L., Lemonnier J.P., Troalen F., Roser J.F., Bousfield G.R., Bellet D., & Bidart J.M. 1993. Immunochemical studies of equine chorionic gonadotropin (eCG), eCG–α and eCG–Β. Endocrinology, 132, 205-211.
  28. Bousfield, G.R., Perry W.M., & Ward, D.N.  1994. Gonadotropins: chemistry and biosynthesis.In: Knobil E., Neill J.D. (ed.) The Physiology of Reproduction, New York: Raven Press, 1749-1792.
  29. Moore K.H., Dunbar B.S., Bousfield G.R., & Ward D.N. 1994. Initial Characterization of Equine Inhibin. Biol. Reprod., 51, 63-71.
  30. Bousfield, G.R. & Ward, D.N. 1994. Evidence for Two Folding Domains in Glycoprotein Hormone Alpha-Subunits. Endocrionology, 135, 624-635.
  31. Matsui, T., Mizuochi, T., Titani, K., Oinaga, T., Hoshi, M., Bousfield, G.R., Sugino, H., & Ward, D.N.  1994. Structural Analysis of N–Linked Oligosaccharides of Equine Chorionic Gonadotropin and Lutropin Beta–Subunits. Biochemistry, 33, 14039-14048.
  32. Robert, P., Troalen, F., Bellet, D., Bousfield, G. R. & Bidart, J.-M. 1994. Immunochemical mapping of human lutropin: I. delineation of a conformational antigenic determinant. Mol. Cell. Endocrinol., 101, 11-20.
  33. Butnev, V.Y., Gotschall, R.R., Baker, V.L., Moore, W.T., & Bousfield, G.R. 1996. Negative Influence of O–linked Oligosaccharides of High Molecular Weight Equine Chorionic Gonadotropin (eCG) on its LH and FSH Receptor Binding Activities. Endocrinology, 137, 2530-2542.
  34. Gotschall, R.R. & Bousfield, G.R. 1996. Oligosaccharide mapping reveals hormone–specific glycosylation patterns on eqiune gonadotropin alpha-subunit Asn56. Endocrinology, 137, 2543-2557.
  35. Butnev, V.Y., Gotschall, R.R., Baker, V.L., Moore, W.T., Gout, P.W. & Bousfield, G.R. 1996. Glycosylated equine prolactin and its carbohydrate moiety. J. Protein Chem., 15, 413-426.
  36. Peng, K.C., Bousfield, G.R., Puett, D., & Ward, D.N.  1996. Circular dichroic spectroscopy of Arg46-nicked ovine lutropin α and derived fragments. J Protein Chem., 15, 547-552.
  37. Bousfield GR, Butnev VY, Gotschall RR, Baker VL, Moore WT:  Structural features of mammalian gonadotropins. Mol. Cell. Endocrinol. 125: 3–19, 1996
  38. Butnev, V,Y., Gotschall, R.R., Butnev, V.Y., Baker, V.L., Moore, W.T., Bousfield, G.R. 1998. Inhibitory influence of alpha-subunit Asn56 oligosaccharide on in vitro subunit association and FSH receptor binding of equine gonadotropins. Biol. Reprod., 58, 458–469.
  39. Bousfield, G.R. 1999. LH (Luteinizing Hormone). In: Knobil, E. and Neill, J.D., (eds.) Encyclopedia of Reproduction, San Diego: Academic Press, Vol. 2, 1034-1054.
  40. Bousfield, G.R., Baker, V.L., Gotschall, RR., Butnev, V.Y., & Butnev, V.Y. 2000. Carbohydrate Analysis of Glycoprotein Hormones. Methods, 21, 15-39.
  41. Bousfield, G.R., Butnev, V.Y. & Butnev, V.Y. 2001. Identifcation of 12 O-glycosylation sites in eLH and eCG alpha subunits by solid-phase Edman degradation. Biol. Reprod., 64, 136-147.
  42. Walton, W.J., Nguyen, V.T., Butnev, V.Y., Singh, V., Moore, W.T., & Bousfield, G.R. 2001. Characterization of Human Follicle-Stimulating Hormone Isoforms Reveals a Non-Glycosylated Beta-subunit In Addition to the Conventional Glycosylated Alpha-subunit. J. Clin. Endocrinol. Metab., 86, 3675-3585.
  43. Butnev, V.Y., Singh, V., Nguyen, V.T., & Bousfield, G.R. 2002. Truncated eLHΒ, Asparagine56-deglycosylated-eLH alpha Combine to Produce a Potent Follicle-Stimulating Hormone Antagonist.  J. Endocrinol., 172, 545-555.
  44. Bousfield, G.R. & Butnev, V.Y. 2002. Equine gonadotropins as models for glycosylation studies. Indian J. Exptl. Biology, 40, 486-506.
  45. Nguyen, V.T., Singh, V., Butnev, V.Y., Gray, C.M., Westfall, S., Davis, J.S., Dias, J.A., & Bousfield, G.R. 2003. Inositol Phosphate Stimulation by LH Requires the Entire Alpha Asn56 Oligosaccharide. Molec. Cell. Endocrinol., 199, 73-86.
  46. Jiang, H., Butnev, V.Y., Bousfield, G.R., & Desaire, H. 2004. Glycoprotein profiling by electrospray mass spectrometry. J. Am. Soc. Mass Spectrom., 15, 750-758.
  47. Bousfield, G.R., Butnev, V.Y., Butnev, V.Y., Nguyen, V.T., Gray, C.M., Dias, J.A., MacColl, R., Eisele, L., & Harvey, D.J.,  2004. Differential Effects of Alpha Asparagine56 Oligosaccharide Structure on Equine Lutropin and Follitropin Hybrid Conformation and Receptor-Binding Activity. Biochemistry, 43, 10817-10813.
  48. Harvey, D.J., & Bousfield G.R., 2005. Differentiation between sulphated and phosphated carbohydrates in low resolution MALDI mass spectra. Rapid Communications in Mass Spectrometry, 19, 287-288.
  49. Bousfield, G.R., Jia, L., & Ward, D.N. 2006. Gonadotropins: Chemistry and Biosynthesis. In:Neill J.D. (ed.) Knobil and Neill: Physiology of Reproduction, 3rd edition. San Diego: Elsevier Press, 1581-1634.
  50. Irungu, J.A., Dalpathado, D.S., Go, E.P., Jiang, H., Ha, H.V., Bousfield, G.R., & Desaire, H., 2006. A Method for Characterizing Sulfated Glycoproteins in a Glycosylation Site-Specific Fashion, Using Ion-Pairing and Tandem Mass Spectrometry. Anal. Chem., 78, 11801-1190.
  51. Dalpathado, D.S., Irungu, J.A., Go, E.P., Butnev, V.Y., Norton, K., Bousfield, G.R., & Desaire, H. 2006. Comparative glycomics of the glycoprotein follicle-stimulating hormone (FSH): Glycopeptide analysis of isolates from two mammalian species. Biochemistry, 45, 8665-8673.
  52. Bousfield, G.R., Butnev, V.Y., Walton, W.J., Nguyen, V.T., Huneidi, J., Singh, V., Kolli, K., Harvey, D.J., & Rance, N.E. 2007. All-or-None N-Glycosylation in Primate Follicle-Stimulating Hormone Beta-Subunits. Mol. Cell. Endocrinol., 260-262, 40-48.
  53. Jablonka-Shariff, A., Roser, J.F., Bousfield, G.R., Wolfe, M.W., Sibley, L.E., Colgin, M., & Boime, I. 2007. Expression And Bioactivity Of A Single Chain Recombinant Equine Luteinizing Hormone (ReLH). Theriogenology,  67, 311-320.
  54. Bousfield, G.R., Butnev, V.Y., Bidart, J.M., Dalpathado, D., Irungu, J., & Desaire, H. 2008. Chromatofocusing fails to separate hFSH isoforms on the basis of glycan structure.Biochemistry, 47, 1708-1720.
  55. Ulloa-Aguirre, A., Dias, J.A,. & Bousfield, G.R. 2009. Gonadotropins and the importance of glycosylation. In: Walsh, G. (ed.) Posttranslational Modifications of Protein Biopharmaceuticals, Wiley-Blackwell, 109-147.
  56. Wehbi, V., Tranchant, T., Durand, G., Musnier, A., Decourtye, J., Piketty, V., Butnev, V.Y., Bousfield, G.R., Crepieux, R., Maurel, M.C., & Reiter, E. 2010. Partially deglycosylated equine LH preferentially activates Beta-arrestin-dependent signaling at the follicle-stimulating hormone receptor. Mol. Endocrinol.,  24, 561-573. 
  57. Visvanathan, M., Siddam, S.R., Lee, I.H., Lushington, G.H., & Bousfield, G.R.  2011. GlycomicsDB – a data integration platform for glycans and their structures. Open Med. Inform. J.,  5, 9-16. 
  58. Bousfield, G.R. & Dias, J.A., 2011. Synthesis and secretion of gonadotropins including structure-function correlates. Rev. Endocr. Metab. Disord., 12(4),  289-302.
  59. Ulloa-Aguirre, A., Dias, J.A., Bousfield, G.R., Huhtaniemi, I. & Reiter, E. 2013. Trafficking of the Follitropin Receptor. Meth. Enzymol., 251, 17-45.
  60. Bousfield, G.R., Butnev, V.Y., Butnev, V.Y., Hiromasa, Y., Harvey, D.J., & May, J.V. 2014. Hypo-glycosylated human follicle-stimulating hormone (hFSH21/18) is much more active in vitro than fully-glycosylated hFSH (hFSH24). Mol. Cell. Endocrinol., 382, 989-997.
  61. Bousfield, G. R., Butnev, V. Y., Rueda-Santos, M. A., Brown, A., Smalter Hall, A., & Harvey, D. J. 2014. Macro and micro heterogeneity in pituitary and urinary follicle-stimulating hormone glycosylation. J. Glycomics Lipidomics, 4, 125.
  62. Davis, J. S., Kumar, T. R., May, J. V., & Bousfield, G. R. 2014. Naturally occurring follicle-stimulating hormone glycosylation variants. J. Glycomics Lipidomics, 4, e117.
  63. Ulloa-Aguirre, A., Reiter, E., Bousfield, G., Dias, J. A., & Huhtaniemi, I. 2014. Constitutive activity in gonadotrophin receptors. Adv. Pharmacol., 70, 37-80.
  64. Bousfield, G. R., Butnev, V. Y., White, W. K., Smalter Hall, A., & Harvey, D. J. 2015. Comparison of follicle-stimulating hormone glycosylation microheterogeneity by quantitative negative mode nano-electrospray mass spectrometry of peptide-N-glycanase-released oligosaccharides. J. Glycomics Lipidomics, 5(1), 129.
  65. Butnev, V. Y., Butnev, V. Y., May, J. V., Shuai, B., Tran, P., White, W. K., & Bousfield, G. R. Production, purification, and characterization of recombinant hFSH glycoforms for functional studies. Mol. Cell. Endocrinol., 405, 41-52.
  66. Cohen, L., Bousfield, G. R., & Ben-Menahem, D. 2015. Recombinant equine LHβ subunit combines divergent intracellular traits of the human LHβ and CGβ subunits. Theriogenology, 83(9), 1469-1476.
  67. Jiang, C., Hou, X., Wang, C., May, J. V., Butnev, V. Y., Bousfield, G. R., & Davis, J. S. 2015. Hypo-glycosylated hFSH has greater bioactivity than fully-glycosylated recombinant hFSH in human granulosa cells. J. Clin. Endocrinol. Metab., 100(6), e852-E860.
  68. Meher, B. R., Dixit, A., Bousfield, G. R., & Lushington, G. H. 2015. Glycosylation effects on FSH-FSHR interaction dynamics: A case study of different FSH glycoforms by molecular dynamics simulations. PLoS ONE, 10(9), e0137897.
  69. Wang, H., Butnev, V. Y., Bousfield, G. R., & Kumar, T. R. 2016. A human FSHB transgene encoding the double N-glycosylation mutant (Asn7Δ Asn25Δ) FSHβ fails to rescue Fshb null mice. Mol. Cell. Endocrinol., 426, 113-124.
  70. Wang, H., May, J., Shuai, B., May, J. V., Bousfield, G. R., & Kumar, T. R. 2016. Evaluation of in vivo bioactivities of recombiant hypo-(FSH21/18) and fully- (FSH24) glycosylated human FSH glycoforms in Fshb null mice. Mol. Cell. Endocrinol., 437, 224-236.
  71. Ulloa-Aguirre, A., Dias, J. A., & Bousfield, G. R. 2017. Gonadotropins. In: Simoni, M. & Huhtaniemi, I. (Eds.) Endocrinology of the Testis and Male Reproduction Springer, Cham., 1-52.
  72. Bousfield, G. R., May, J. V., Davis, J. S., Dias, J. A., & Kumar, T. R. 2018. In vivo and in vitro impact of carbohydrate variation on follicle-stimulating hormone function. Front. Endocrinol., 9, 216.
  73. Bousfield, G. R. 2019. Biosynthesis and Posttranslational Processing of Peptide Hormones.Reference Module in Biomedical Sciences., 

    doi: 10.1016/B978-0-12-801238-3.92822-8.

  74. Bousfield, G. R. & Harvey, D.J. 2019. Follicule-Stimulating Hormone Glycobiology.Endocrinology, 160(6), 1515-1535.

 

Professional Experience

AnchorProfessional Experience

Year(s) Position Held
1974 - 1977 Associate Instructor, Indiana University
1978 - 1979 Predoctoral Fellowship, Robert A. Welch Foundation, The University of Texas M.D. Anderson Hospital and Tumor Institute, Houston,TX
1979 - 1981 Research Assistant, The University of Texas M.D. Anderson Hospital and Tumor Institute, Houston, TX
1981 - 1983 Postdoctoral Fellowship, Robert A. Welch Foundation, The University of Texas M.D. Anderson Hospital and Tumor Institute, Houston, TX
1986 Visiting Scientist, National Institute of Immunology, New Dehli, India
1983 - 1987 Research Associate, The University of Texas M.D. Anderson Hospital and Tumor Institute, Houston, TX
1988 - 1990 Assistant Biochemist and Assistant Professor of Biochemistry and Molecular Biology (non-tenured research appointment), M.D. Anderson Cancer Center, Houston, TX
1990 Visiting Scientist, Institute of Physical and Chemical Research, RIKEN, Wako, Japan
1991 Visiting Assistant Professor, Department of Biological Sciences, Wichita State University, Wichita, KS
1991 - Present Director, Protein Chemistry Core Laboratory, Department of Biological Sciences, Wichita State University, Wichita, KS
1992 - 1997 1992-1997 Assistant Professor, Department of Biological Sciences, Wichita State University, Wichita, KS
1994  Ad hoc member, NIH DRG, Biochemical Endocrinology Study Section
1994 & 1995 NIH, NICHD site visit
1995 - 2001 Scientist, Women’s Research Institute, University of Kansas Medical School at Wichita, Wichita, KS
1997 - 2005 Associate Professor, Department of Biological Sciences, Wichita State University, Wichita, KS
2005 - 2010 Professor, Department of Biological Sciences, Wichita State University, Wichita, KS
2010 - present Lawrence M. Jones Distinguished Professor, Department of Biological Sciences, Wichita State University, Wichita, KS

 

Awards and Honors

Professional Organizations and Honors

  • Sigma Xi
  • American Association for the Advancement of Science
  • American Chemical Society
  • American Society for Biochemistry and Molecular Biology
  • The Endocrine Society
  • The Protein Society
  • Society for the Study of Reproduction
  • Society for Glycobiology
  • Association of Biomedical Resource Facilities
  • 1974, William B. Owsley Outstanding Student in Biology Award, Presented by Saginaw Valley State College
  • 1994 Wichita State University Mortar Board, Educator Appreciation Award
  • 2003 Kansas Biomedical Research Infrastructure Network Faculty Scholar Award
  • 2010 Wichita State University Excellence in Research Award
Grants

 Awarded Grants

Period Grant Awarded
12/01/1989 - 11/30/1990 Carbohydrate Chemistry of Equine Gonadotoropins. M.D. Anderson Cancer Center Institutional Research Grant.
10/01/1996 - 08/31/1996 Role of Carbohydrate in FSH Specificity Determination.  National Institutes of Health, HD 29047.
01/01/1994 - 06/30/1994 Chimeric Gonadotropin Alpha Subunits. Wichita State University, University Research Grant.
08/01/1997 - 07/31/2000 Functional Consequences of Carbohyrate Differences Between eLH and eCG. National Institutes of Health, DK 52383.
02/01/2000 - 01/31/2001 Acquisition of a Protein Sequencer, NSF EPSCoR.
07/01/2001 - 06/30/2002 Adaptation of Adsorptive Sequencer to Solid-Phase Edman Degradation, Wichita State University, University Research Grant
07/01/2002 - 06/30/2003 Carbohydrate Modulation of Gonadotropin Action. National Institutes of Health/KUMC-KBRIN RR-16475, Joan Hunt, PI.
09/30/2002 - 08/31/2005 Structural Characterization of Glycoproteins using Mass Spectrometry. (Sub-contract, Heather Desaire, KU, PI) National Institutes of Health/KU-COBRE RR-017708 Bob Hanzlik PI
12/15/2002 - 08/31/2003 Replacement of Dionex Carbohydrate Analyzer Detector. National Institutes of Health/KUMC-KBRIN RR-16475, Joan Hunt PI.
09/01/2003 - 06/30/2004 Replacement of Dionex Carbohydrate Analyzer Detector. National Institutes of Health/KUMC-KBRIN RR-16475, Joan Hunt PI.
10/01/2003 - 09/30/2004 Follicle-Stimulating Hormone Antagonist Inhibition of Prostate Tumor Cell Division, Flossie E. West Memorial Foundation.
12/19/2003 - 06/30/2004 Replacement Of Dionex High pH Liquid Chromatograph For Carbohydrate Analysis. National Institutes of Health/KUMC-KBRIN RR-16475, Joan Hunt PI.
03/01/2004 - 02/28/2005 Acquisition of Fluorescence and Phospho Imaging Instruments, NSF-EPSCoR, EPS-0236913, Thomas N. Taylor, Project Director.
07/01/2005 - 06/30/2006 Procise sequencer upgrade. National Institutes of Health/KUMC-KBRIN RR-16475, Joan Hunt PI.
04/01/2005 - 03/31/2006 Glycan Inhibition of LH and FSH Signaling. National Institutes of Health/KUMC-KBRIN RR-16475, Joan Hunt PI.
09/01/2005 - 12/31/2006 Gonadotropin Characterization, Serono, Inc., Rockland, MA,  Research Contract.
08/01/2008 - 12/31/2009 Monoclonal Antibody Production for Follicle Stimulating Hormone Glycoforms, Kansas Technology Enterprise Corporation
03/01/2009 - 05/31/2022 Aging Pituitary/Gonadal Axis, National Institutes of Health/NIA, P01AG029531

 

Additional Information

AnchorEducation

Institution and Location Degree Year Field of Study
Saginaw Valley State College, University Center, MI B.S. 1974 Chemistry & Biology
Indiana University, Bloomington, IN M.A. 1976 Zoology
Indiana  University, Bloomington, IN Ph.D. 1981 Zoology