Shang-You Yang PhD

Research Interests

Research Projects in Progress:

1. SCID mice model to study the interactions among peripheral blood monocytes, human periprosthetic tissue and bone tissue that lead to wear debris-associated loosening following total joint replacement.
2. Combination gene therapy of anti-inflammation and counter-osteolysis for rheumatoid arthritis using a mouse collagen-induced arthritis model.
3. A long-term murine model of prosthetic knee joint for implant stability, debris-associated bone resorption and potential gene therapy.
4. Soluble VEGF receptor gene therapy in controlling growth and metastasis of osteosarcoma in a novel mouse model.
5. Engineered bone graft substitutes for large bone defect. The study uses in intro and in vivo approaches to explore the type, composition, porosity, and biocompatibility of biomaterial scaffolds with the bedding and growth of osteo- and endothelial progenitor cells directional inducted from bone marrow stromal cells.
6. Minocyclin-associated bone discoloration and its molecular mechanism.

Publications

Peer-Reviewed Papers (selected from last 6 years):

1. Yu H., P.J. VaneVord, L Mao, H.W. Matthew, P.H. Wooley, S-Y Yang (2008) Improved tissue-engineered bone regeneration by endothelial cell mediated vascularization. Biomaterials doi:101016/j.biomaterials:2008.09.047
2. D. Yin, T. Jia, H. Yu, P.H. Wooley, M.P. Mott, S-Y Yang. (2008) VEGF Blockade Decelerates the Growth of a Murine Experimental Osteosarcoma. Int J Oncol. 2008 Aug;33(2):253-9.
3. Yu H., B. Wu, L. Mayton, Z. Song, H.W. Matthew, P.J. VandeVord, P.H. Wooley, S-Y Yang(2008) Promotion of osteogenesis in tissue engineered bone by preseeding endothelial progenitor cells-derived endothelial cells. J Orthop Res. 2008 Aug;26(8):1147-52.
4. Zhang L. Niu T. Yang S-Y. Chen B (2008). The occurrence and regional distribution of DR4 on herniated disc cells: a potential apoptosis pathway in lumbar intervertebral disc. Spine. 2008 Feb; 33(4):422-7.
5. Yu, H., H. W. Matthew, P. H. Wooley, and S.-Y. Yang. (2008) Effect of Porosity and Pore Size on Microstructures and Mechanical Properties of Poly-ƒÕ-Caprolactone-Hydroxyapatite Composites. J Biomed Mater Res B. 2008 Aug;86B(2):541-7.
6. Yang S-Y, H Yu, J E Krygier, P H Wooley, M P Mott. (2007) High VEGF with Rapid Growth and Early Metastasis in a Mouse Osteosarcoma Model. Sarcoma 2007: 95628, 7 pages, doi:10.1155/2007/95628.
7. Zhang, T.; Gong, W.; Yang, S.; Zhang, K.; Yin, D.; Xu, P.; Jia, T. (2007) Efficacy of Hyperbaric Oxygen on Survival of Random Pattern Skin Flap in Diabetic Rats. Undersea Hyperb. Med. 2007, 34, 335-339.
8. Yang, S.-Y., H. Yu, B. Wu, L. Mayton, R Costello, and P. H. Wooley (2007) A Murine Model of Prosthesis Failure for the Long-term Study of Aseptic Loosening. J Ortho Res 2007 May;25(5):603-11.
9. Shang-You Yang and Paul H. Wooley (2006) Murine Air Pouch Model of Inflammation to Evaluate Orthopaedic Wear Debris Associated Inflammation and Potential Inhibitory Cytokine Gene Therapy. pp 155-168, Progress in Inflammation Research (Editor: Pitzer, James A)” ISBN: 1-60021-008-2
10. Yang, S.-Y., B. Wu, L. Mayton, S. Nasser, D. C. Markel, and P. H. Wooley (2005) IL-1ra gene transfer attenuates the inflammation of human periprosthetic tissues. J Bone & Joint Surg –[AM] 87(5):1088-1097.
11. P. Mukherjee, S.-Y. Yang, B. Wu, Z. Song, L.K. Myers, P.D. Robbins, P.H. Wooley (2005) Tumor necrosis factor receptor gene therapy affects cellular immune responses in collagen induced arthritis in mice Ann Rheum Dis. 64:1550-1556
12. W. Ren*, S-Y. Yang*, P. H. Wooley (2004) A novel model of orthopaedic wear debris – associated osteolysis. *Equal contribution Scand.J.Rheumatol 33(5):349-357.
13. Kallakuri S, Takebayashi T, Ozaktay AC, Chen C, Yang S, Wooley PH, Cavanaugh JM. (2004) The effects of epidural application of allografted nucleus pulposus in rats on cytokine expression, limb withdrawal and nerve root discharge. Eur Spine J. 2004 Jul 28; [Epub ahead of print].
14. Yang, S.-Y., B. Wu, P. Mukherjee, L. Mayton, C. H. Evans and P. H. Wooley (2004) Protective effects of IL-1Ra or vIL-10 gene transfer on a murine model of wear debris-induced osteolysis. Gene Therapy 11(5):483-491 .
15. W. Ren, S-Y. Yang, Y. Park, S. Hsu, and P. H. Wooley (2003) Receptor activator of NF-ƒÛB (RANK) and RANK ligand (RANKL) expression in response to varying morphology of UHMWPE particles in a murine model of inflammation Biomaterials. 24(26):4819-4826
16. S.-Y. Yang, L. Mayton, B. Wu, J. J. Goater, E. M. Schwarz, and P. H. Wooley (2002) Adeno-Associated Virus-Mediated Osteoprotegerin Gene Transfer Protects against Particulate Polyethylene-Induced Osteolysis in a Murine Model Arthritis & Rheumatism 46(9):2514-2523.
17. Yang, S-Y; W. Ren; Y. Park; S. Hsu; S. Nasser; and P. H. Wooley (2002) Diverse Cellular and Apoptotic Responses to Variant Shapes of UHMWPE Particles in a Murine Model of Aseptic Loosening Biomaterials 23(17):3535-3543
18. Yang S.-Y., B. Wu, L. Mayton, C. H. Evans, P. D. Robbins, S. Nasser and P. H. Wooley (2002) Retrovirus-Mediated IL-10 And IRAP Gene Transfer Ameliorate The Local Inflammatory Reaction In A Murine Model Of Inflammation. Inflamm. Res. 51(7): 342-350
19. Y.S. Park, S.Y. Yang, P.H. Wooley, K. Merritt, S. Hsu, M.J. McNallan (2001) Bioactivity Responses of Different UHMWPE Particles from In-vivo and In-vitro Tests. Mat.Res.Soc.Symp.Proc. 662:381-386
20. Sud, S., S.-Y. Yang, C. H. Evans, P. D. Robbins and P. H. Wooley (2001) Effects of cytokine gene therapy on particulate-induced inflammation in the murine air pouch. Inflammation 25: 361-372
21. Wooley, P. H., R. Morren, J. Andary, S. Sud, S.-Y. Yang, L. Mayton, D. Markel and S. Nasser (2002) Inflammatory responses to orthopaedic biomaterials in the murine air pouch. Biomaterials 23(2):517-526

Published Abstracts (last 3 years)
1. S.-Y. Yang, Bin Wu, Lois Mayton, Paul H. Wooley (2004) Local Gene Transfer of OPG Prevents Joint Damage and Disease Progression in Collagen-induced Arthritis Arthritis Rheumat, 50:9(suppl):334
2. S.-Y. Yang; B. Wu; L. Mayton; R. Costello; H. Yu; and P. H. Wooley (2005) A murine model of knee joint prosthesis failure for long-term study of aseptic loosening and potential gene therapy. Orthopaedic Research Society Transactions, Vol.30:1220
3. H. Yu, S.-Y. Yang, P. H. Wooley (2005) Pore Size of Hydroxyapatite/Agarose Gel Influences Viability of Bone Marrow Stromal Cells. Transactions of Society for Biomaterials, Vol 30: 410
4. S.Y. Yang, B. Wu, L. Mayton, P. H. Wooley (2005). A SCID-Hu Mouse Model for Osteolytic-Associated Prosthetic Joint Loosening Arthritis Rheumat, 52:9(suppl):S701
5. H.-N. Hao, S.-Y. Yang, P. H. Wooley (2005) Direct interaction of collagen II and toll-like receptor depend upon the saccharide residues of collagen II: a potential mechanism for collagen-induced arthritis. Arthritis Rheumat, 52:9(suppl):S477
6. H. Yu, P. H. Wooley, S.-Y. Yang (2006) Biocompatibility of PCL-HA hybrid with bone marrow derived osteogenetic and vasculogenetic cells. Orthopaedic Research Society Transactions, Vol.31: 830
7. Hao, H-N; Silver, R; Zheng, B; Yang, S-Y; Ren, W; Nasser, S; Wooley, P (2006) The roles of monocytic heat shock protein 60 and toll-like receptor in response to wear debris particles in aseptic loosing. Orthopaedic Research Society Transactions, Vol.31: 399
8. H. Yu, H.W. Mathew, C.D. Huber, P. H. Wooley, S.-Y. Yang (2006) Effect of porosity and pore size on microstructures and mechanical properties of poly-a-caprolactone (PCL)-hydroxyapatitie (HA) composites. Transactions of Society for Biomaterials, Vol 31: 499
9. S.-Y. Yang, J. Krygier, P. H. Wooley, M. P. Mott. High VEGF Expression Associates with Early Tumor Development and Fast growth in a Novel Model of Osteosarcoma. (2006) Transactions for Musculoskeletal Tumor Society2006: 43
10. S-Y Yang, B Zheng, H Yu, P D Robbins, H-N Hao, and P H Wooley. (2006) OPG or sTFNR Gene Transfer Respectively Ameliorates the Disease Progression without Synergic Effects in Collagen-induced Arthritis Arthritis Rheumat, 54:9(suppl):S242
11. Yu, H; Bin, W; Mayton, L; Song, Z; Wooley, P H; Yang, S-Y. (2007) Promote osteogenesis in tissue engineered bone by pre-seeding endothelial progenitor cells-derived endothelial cells. Orthopaedic Research Society Transactions, Vol.32: 347
12. Yang, S-Y; Yu, H; Wu, B; Krygier, J; Mott, M; Wooley, PH. (2007) High VEGF expression associates with fast tumor development and early lung metastasis in a murine model of Osteosarcoma. Orthop. Res. Soc. Transactions, Vol.32: 0983
13. H Yu; B Wu; L Mayton; Z Song; P. H. Wooley; S.-Y. Yang. (2007) Pre-Seeding Bone Marrow Cells-Derived Endothelial Cells Promotes in vivo Osteogenesis of Bone Substitutes Transactions of Society for Biomaterials, Vol 32: 61
14. H-N Hao, S-Y Yang, P. H. Wooley. (2007) Toll-Like Receptor Expression is Necessary for Synovial Type II Cells to Respond to LPS: a Potential Mechanism of Reactive Arthritis J. Immunol., 178: 101.14.
15. K. Zhang, T-H Jia, D. Yin, T. Zhang, D. Markel, P. H. Wooley, and S-Y Yang. (2008) A SCID-Hu Chimera Model with Peripheral Blood Monocytes Transfusion for Osteolytic Prosthesis Failure. Orthop. Res. Soc. Transactions Vol.33:1941.
16. H. Yu, H.W. Matthew, P.J. VandeVord, P.H. Wooley, S-Y Yang (2008) Improve Tissue-Engineered Bone Regeneration by Introducing Endothelial Cells-Initiating Vascularization. Tissue Engineering International & Regenerative Medicine Society (TERMIS_EU) Porto, Portugal 2008
17. K Zhang, D-Z Yin, T Jia, T Zhang, P Xu, H-N Hao, P Wooley, S-Y Yang. (2008) PMMA and Ti Particles Promote Blood Monocytes Trafficking and Local Inflammation in a Mouse-Hu Chimera of Aseptic Loosening. Arthritis Rheumat, 58:9(suppl):S1243

Professional Experience

• 06/2008 – now Senior Research Scientist Orthopaedic Research Institute, Via Christi Health System, Wichita, KS
• 10/2007 – now Guest Professor Shandong University Affiliated Jinan Central Hospital, Jinan, China
• 09/2002 – 06/2008 Assistant Professor Department of Orthopaedic Surgery, School of Medicine, Wayne State University
• 01/2003 – 06/2008 Assistant Professor Department of Biomedical Engineering, College of Engineering, Wayne State University
• 03/1990 – 02/1994 Research Associate University of Manitoba, Winnipeg, Canada
• 08/1988 – 03/1990 Staff Surgeon in Orthopaedics Jinan Municipal Central Hospital, Shandong Province, China

Current Grant Support:

• 2007 - 2010 National Institute of Health (NIAM, 1R03AR054929): The study of gene therapy for aseptic loosening using a mouse implant model. Principle Investigator (PI)
• 2008-2010 Orthopaedic Research and Education Foundation (OREF): “Cell Trafficking in Debris-Associated Osteolysis”, PI
• 2008 – 2011 National Science Foundation (NSF) Developing a Biomimetric Approach Using a Hydroxyapatite-Collagen Composite for Load Bearing Orthopaedic Applications (PI: Dr. Pam VandeVord). Co-I